Use of the Delphi Panel Method to Assess Expert Perception of the Accuracy of Screening Test Systems for Infectious Pancreatic Necrosis Virus and Infectious Hematopoietic Necrosis Virus

1999 ◽  
Vol 11 (2) ◽  
pp. 139-147 ◽  
Author(s):  
N. Nathalie Bruneau ◽  
Margaret A. Thorburn ◽  
Roselynn M. W. Stevenson
Keyword(s):  
Screening Test ◽  
Pancreatic Necrosis ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Infectious Pancreatic Necrosis Virus ◽  
Delphi Panel ◽  
Necrosis Virus ◽  
Test Systems ◽  
Infectious Hematopoietic Necrosis ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Fish Cell Lines: Comparisons of CHSE-214, FHM, and RTG-2 in Assaying IHN and IPN Viruses

1978 ◽  
Vol 35 (7) ◽  
pp. 1009-1011 ◽  
Author(s):  
R. K. Kelly ◽  
B. W. Souter ◽  
H. R. Miller
Keyword(s):  
Chinook Salmon ◽  
Fathead Minnow ◽  
Pancreatic Necrosis ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Infectious Pancreatic Necrosis Virus ◽  
Fish Cell ◽  
Necrosis Virus ◽  
Infectious Hematopoietic Necrosis ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

The rainbow trout, Salmo gairdneri, gonad (RTG-2) cell line was more sensitive to lytic infection by infectious pancreatic necrosis virus (IPNV) than was the fathead minnow, Pimephales promelas, (FHM) cell line in laboratory and field studies. Laboratory investigations with known strains of IPNV (VR 299 and Buhl) also indicated that the cytopathic response in IPNV-infected chinook salmon, Oncorhynchus tshawytscha, embryo (CHSE-214) cells was greater than in infected FHM cells. In contrast, titration experiments with standard infectious hematopoietic necrosis virus (IHNV) isolates (Oregon and Cedar River) showed that the FHM cells were more sensitive to destructive infection by IHNV than was either of the salmonid cell lines (RTG-2, CHSE-214). Key words: fish cell culture, infectious pancreatic necrosis virus, infectious hematopoietic necrosis virus, chinook salmon embryo cells, fathead minnow cells, rainbow trout cells, salmonid viruses

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